Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies against Chlamydia trachomatis.
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چکیده
منابع مشابه
Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies against Chlamydia trachomatis.
An enzyme linked fluorescence immunoassay (ELFA) has been evaluated for the detection of antibodies against Chlamydia trachomatis. Reticulate bodies and elementary bodies from C trachomatis L2/434 strain were used as antigens. An enzyme linked immunosorbent assay (ELISA) has also been evaluated using the same antigens. Results obtained by ELISA and ELFA for human sera with these two antigens we...
متن کاملIgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay
A simple, sensitive enzyme linked fluorescence immunoassay has been developed to detect IgM antibodies against Chlamydia trachomatis. Reticulate bodies and elementary bodies from C trachomatis L/434 Bu strain were isolated and used as antigens in the assay. Of 113 serum samples obtained from infants with pneumonia, 27 (23.9%) had IgM antibodies to C trachomatis L2 reticulate bodies and nine (8....
متن کاملDetection of IgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay.
A simple, sensitive enzyme linked fluorescence immunoassay has been developed to detect IgM antibodies against Chlamydia trachomatis. Reticulate bodies and elementary bodies from C trachomatis L2/434 Bu strain were isolated and used as antigens in the assay. Of 113 serum samples obtained from infants with pneumonia, 27 (23.9%) had IgM antibodies to C trachomatis L2 reticulate bodies and nine (8...
متن کاملComparison of reticulate and elementary body antigens in detection of antibodies against Chlamydia trachomatis by an enzyme-linked immunosorbent assay.
Reticulate bodies from a type C and elementary bodies from a type L2 strain of Chlamydia trachomatis were isolated and used as antigens in an enzyme-linked immunosorbent assay (ELISA). Results obtained for human sera with these two antigens used in the ELISA were compared with each other and with results obtained for the same sera by the micro-immunofluorescence test. Negative control populatio...
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An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1985
ISSN: 0021-9746
DOI: 10.1136/jcp.38.3.345